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KMID : 0613820030130060933
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Journal of Life Science
2003 Volume.13 No. 6 p.933 ~ p.942
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Restoration of Saccharomyces cerevisiae coq7 Mutant by a Neurospora crassa Gene
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Kim Eun-Jung
Kim Sang-Rae
Lee Bheong-Uk
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Abstract
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CoenzymeQ is a quinone derivative with a long isoprenoid side chain. It transports electrons in the respiratory chain located in the inner mitochondrial membrane of eukaryotes and the plasma membrane of prokaryotes. It also functions as an antioxidant. Saccharomyces cerevisiae coq mutants, that are deficient coenzyme Q biosynthesis fail to aerobically grow. They are not able to grow on non-fermentable carbon sources, such as glycerol, either. The putative coq-7 gene involved in coenzyme Q biosynthesis of Neurospora crassa was cloned and used for complementation of S. cerevisiae coq7 mutant. The predicted amino acid sequence of N. crassa COQ7 showed about 58% homology with Coq7p of S. cerevisiae. The growth rate of S. cerevisiae coq7 mutant transformed with the N. crassa coq-7 gene was restored to the wild-type level. The complemented S. cerevisiae strain was able to grow with glycerol as a sole carbon source and showed less sensitivities to linolenic acid, a polyunsaturated fatty acid.
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KEYWORD
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Neurospora crassa, COQ7, coenzyme Q, Ubiquinone, antioxidant, Saccharomyces cerevisiae
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